Perioxidase test to detect elevated concentrations of leuocytes

Elevated concentrations of leukocytes in semen have been associated with genital tract infection, poor semen quality and IVF/embryo transfer failure.

Leukopcytes, especially polymorphic polynuclear leukocytes (PML), are present in most human ejaculates. By normal microscopy these cells can be morphologically easily mixed up with multi-nuclear spermaids.

By using hydrogen peroxide (H2O2) peroxidase-positive leukocytes (neutrophils polymorphic granulocytes) can be stained yellow to brown. Other cells (sperm, lymphocytes, monocytes, macrophages and multinucleated spermatids) remain unstained (peroxidase-negative). With this kit the seminal fluid is treated with the reagents 1 and 2 in which only peroxidase stainpositive cells remain brown making the leukocytes easier to identify using phase contrast microscopy.

 

Product description
Product code
Unit
SpermLeu Reagents 1 & 2 FGYZR103020

Kit contains

1 x 20ml Reagent 1

1 x 1ml Reagent 2

 24 month shelf life

Must be stored at 2-8 oC

Contact Info

Planer Limited
110 Windmill Road
Sunbury-On-Thames
Middlesex, TW16 7HD
United Kingdom

Telephone: +44 1932 755000
email: enquiries@planer.com
Service: service@planer.com
Orders: orders@planer.com