Perioxidase test to detect elevated concentrations of leuocytes
Elevated concentrations of leukocytes in semen have been associated with genital tract infection, poor semen quality and IVF/embryo transfer failure.
Leukopcytes, especially polymorphic polynuclear leukocytes (PML), are present in most human ejaculates. By normal microscopy these cells can be morphologically easily mixed up with multi-nuclear spermaids.
By using hydrogen peroxide (H2O2) peroxidase-positive leukocytes (neutrophils polymorphic granulocytes) can be stained yellow to brown. Other cells (sperm, lymphocytes, monocytes, macrophages and multinucleated spermatids) remain unstained (peroxidase-negative). With this kit the seminal fluid is treated with the reagents 1 and 2 in which only peroxidase stainpositive cells remain brown making the leukocytes easier to identify using phase contrast microscopy.
|SpermLeu Reagents 1 & 2
1 x 20ml Reagent 1
1 x 1ml Reagent 2
24 month shelf life
Must be stored at 2-8 oC