For distribution in the UK only

Find the fittest - MACS® ART Annexin V System

Easy, fast, and specific depletion of unwanted apoptotic spermatozoa

• Improve quality of sperm preparations
• Obtain desired population within 30 minutes
• Proven technology for optimal results

Improve the quality of spermatozoa preparations Be specific, be successful The presence of apoptotic cells in sperm preparations for assisted reproduction is known to decrease specimen quality. Unfortunately, commonly used techniques for preparing sperm may not remove apoptotic spermatozoa with the required specificity. That’s why the MACS® ART Annexin V System was designed to selectively remove apoptotic spermatozoa from sperm preparations. Even early apoptotic spermatozoa with an intact cellular membrane are efficiently depleted, to yield spermatozoa fractions for use in subsequent operations in Assisted Reproductive Technology (ART). Select only the highest quality for your next sperm preparation.

  • Benefits +

    Researchers have reported that purified sperm populations show higher overall quality in terms of:

    • Morphology¹
    • Sperm Deformity Index¹
    • Motility²,³
    • DNA fragmentation
    • Mitochondria membrane potential integrity³,⁴,⁵
    • Apoptosis
    • Cryosurvival rates²,⁴

    Scientist obtained a sperm population with:

    • Improved sperm fertilization potential (sperm capacitation index, hamster oocyte penetration assay)⁶,12
    • Improved Induced Acrosome Reaction Test (IART)⁷,⁸
    • Increased sperm chromatin decondensation⁹,11
    • Improved cleavage rates after fertilization¹⁰
  • References +

    1 Aziz, N. et al. (2007) Human Reproduction 22: 1413–1419.
    2 Said, T. et al. (2005) Fertility and Sterility 83: 1442–1446.
    3 De Vantéry Arrighi, C. et al. (2009) Reproductive Biology Endocrinology 7: 1.
    4 Grunewald, S. et al. (2006) Cell and Tissue Banking 7: 99–104.
    5 Paasch, U. et al. (2005) Asian Journal of Andrology 7: 61–69.
    6 Said, T. et al. (2006) Biology of Reproduction 74: 530–537.
    7 Lee, T. et al. (2010) Human Reproduction 25: 839–846.
    8 Grunewald, S. et al. (2006) Ann. N. Y. Acad. Sci. 1090: 138–146.
    9 Grunewald, S. et al. (2009) Fertility and Sterility 92: 572–577.
    10 Dirican, E. K. et al. (2008) Journal of Assisted Reproduction and Genetics 25: 375–381.
    11 Delbes, G. et al. (2013) Journal of Andrology 1: 689–706.
    12 Sheikhi, A. et al. (2013) Journal of Andrology 1: 845–849.

  • How it works +

    Apoptotic cell depletion begins with magnetic labeling of apoptotic cells by the MACS ART Annexin V Reagent. The labeled cells are then passed through a separation column
    located in a magnetic field. Unwanted cells are selectively retained in the column. Living spermatozoa are not labeled by the reagent, so they pass through the column and are
    collected for later use.

     Figure 1: Translocation of PS from the intracellular to the extracellular surface of the plasma membrane during apoptosis.

    Figure 2: Isolation of PS-exposing cells using the MACS ART Annexin V System.

  • Downloads +

    Specification Sheet

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