Planer will once again be exhibiting at the European Society of Human Reproduction and Embryology (ESHRE) conference in 2018. This year the conference is being held at the Centre de Convencions Internacional de Barcelona (CCIB) in Barcelona, Spain from the 1st to the 4th July.
We would welcome any of our distributors and their customers to come and see us at this year’s conference where we will have the CT37stax™ multi chamber incubator and DATAssure™alarm and monitoring system on display alongside a range of cryogenic storage vessels suitable for the ART market.
We look forward to seeing many of you at this important event in the ART calendar. Please do let us know if you are planning on attending - we will be on stand 386.
This year, the biennial meeting of ALPHA, the international society aimed at advancing the science of clinical embryology around the world, will be taking place in the beautiful city of Reykjavik, Iceland between 17th and 20th May. Planer will be attending the meeting – if you are going, please do come and find out more about our latest range of products.
CT37stax™ multi chamber incubator
Our CT37stax™ incubator complements the existing range of well-established Planer benchtop incubators. It is a high capacity incubator, which has been designed using the best features of the existing technology and incorporates these with a mix of innovative new features to provide a state of art new multi chamber benchtop incubator fit for the demands of the modern laboratory.
We are delighted to announce that the CT37stax™ has just gained medical device accreditation under the Medical Devices Directive 93/42/ECC - which now allows this product to be sold for human applications e.g. ART applications in all countries covered by these regulations.
DATAssure™ wireless monitoring system
The Planer DATAssure™ wireless monitoring system is used across a broad range of market sectors meeting the most stringent standards to assist customers to comply with HACCP, BRC, FDA and MRHA legislative requirements. The system is standalone and connects directly to your business IT network with no need for any dedicated PC, server or specialist installed software. Data from the base station can be viewed direct on the colour touch screen or via a standard web browser.
Our ShipsLog3™ is a temperature data logger that provides an accurate and downloadable temperature history of your vapour shipper throughout its transit. It measures and stores more than 32,000 temperature readings from the thermocouple fitted to the inside of the shipper lid. This will monitor and record the temperature of the shipper at the warmest part ensuring the samples in the core of the shipper are stored at the correct temperature and providing early alerts of potential warming issues.
If you are unable to attend ALPHA, but would like to find out more about our range of products suitable for the clinical embryology field, please do get in touch. We would be delighted to hear from you.
A study on these freezing responses, relating to aggregates versus single cells, has just been published in Tissue Engineering (on-line February 2018) by Rui Li, Guanglin Yu (pictured here) and others working with Prof Allison Hubel's team at the Department of Mechanical Engineering University of Minnesota. Human induced pluripotent stem cells (hiPSCs) are multicellular aggregates attracting much interest in tissue engineering, disease modelling and personalised medicine. They can be frozen either as aggregates or single cells depending upon the application. For both clinical and scientific purposes, effective cryopreservation of hiPSCs is required for transportation, storage of frozen hiPSCs and other downstream uses. However, cryopreserved hiPSCs are vulnerable to the loss of viability, function or pluripotency.
It is known that inadequate preservation methods of hiPSCs have impeded efficient re-establishment of cell culture after their freeze-thaw. In the study the roles of cooling rate, seeding temperature and the difference between cell aggregates and single cells in controlled rate freezing were examined using, inter alia, Raman spectroscopy, as a tool for understanding cell responses to the freezing environment. The Raman spectroscopy was used to observe both hiPSC single cells and aggregates frozen at three cooling rates and two seeding temperatures; it suggested higher sensitivity of aggregates to supercooling than previously thought. The work will deepen understanding of behaviours of single cells and aggregates frozen at various conditions and help promote the development of improved cryopreservation protocols for human induced pluripotent stem cells.
For single cells, slow cooling rates allowed significantly better preservation of membrane integrity than higher cooling rate (10˚C/min) regardless of the seeding temperature. For aggregates, however, slow cooling rates (1, 3˚C/min) combined with high seeding temperature (4˚C) had little effect on the membrane integrity but resulted in significantly better cell attachment than higher cooling rate (10˚C/min) or low seeding temperature (8˚C). The authors say there are advantages of using a seeding temperature of 4˚C compared to 8˚C suggesting that the range of seeding temperatures of 7˚C to 12˚C quoted in much literature may be sub-optimal, and that seeding temperature should be considered as a critical parameter when designing cryopreservation protocol for hiPSCs. Guanglin Yu, see photo above, carried out a lot of the experimentation. He said "...we used manual seeding for nucleation of the sample. We sprayed the sample with a narrow stream of liquid nitrogen. We saw different cell responses of HiPSC with seeding temperature of -4˚C and -8˚C using Raman microscopy. This made it clear that we wanted to control the temperature at which ice formed in the extracellular space for controlled rate freezing experiments."
The paper indicates that hiPSCs respond to freezing in very complex fashion, and successful establishment of post thaw culture depends on various critical factors. Further studies will need to not only continue exploring additional factors to optimise the freezing protocol for hiPSCs but investigate the biological pathways connecting the factors and the observed cryopreservation outcomes to provide targets for future development of cryoprotectants.
For further information
Tissue Engineering: https://www.liebertpub.com/doi/abs/10.1089/ten.TEC.2017.0531
Planer Controlled Rate Freezer: https://planer.com/products/cryo-freezers/medium-crf.html
On the 34th anniversary of the birth of the first baby from a frozen embryo, the BBC World Service broadcast an interview with Alan Trounson the pioneer doctor who undertook the procedure.
Zoe Leyland was born in Melbourne, Australia on 28th March 1984, helped on her way by Drs Trounson and Wood who made medical history. The decision to try 'test tube' fertilisation and embryo freezing was taken by Zoe's parents – her mother a 33 year old New Zealander and father a 38 year old British born Australian resident. Her mother had hormonal stimulation and produced eleven eggs which were frozen using a then new type of controlled rate freezer made by Planer plc. One of those frozen embryos became Zoe - who weighed in at about 5 lbs or 2.5 kilos.
The world's first 'fresh’ test tube baby was Louise Brown born in England in 1978, but Zoe came from an embryo frozen for a time before being thawed and implanted. To allow cells to survive liquid nitrogen temperatures (-196°C) the embryos had to be treated with cryo-protectant, then slowly frozen down in the Planer freezer with extreme precision using different temperature ramps, before being stored in liquid nitrogen. This controlled rate freezing procedure is now used in cell laboratories worldwide. The controlled rate freezing technique, originally suggested some fifty years ago by British Scientist Professor David Pegg, enabled Planer plc to pioneer the equipment and today many thousands of units are in constant use all over the world in stem cell labs, IVF, hospitals and research institutions. This controlled rate freezing is often needed before storing certain types of cells in liquid nitrogen – in areas such as cord blood banking, bone marrow transplants, botanical matter, semen, oocytes, botanical seeds, skin, ovarian tissue, heart valves and blood vessels.
The AVA Clinic in Latvia was opened in 2005. The experienced team get high infertility treatment results, and as they adhere strictly to ISO 9001:2008 , the same rigour must apply to safety in their cryo store - which is why they chose our O2NE+ low oxygen alarm system.
In the unlikely event that there were to be a leak in the cryo store causing a build-up of nitrogen gas, which is colourless and odourless, the depletion of oxygen levels might pose a serious danger. The O2NE+ system provides two audio visual alarms which are usually pre-set at 19.5% & 18% to warn personnel of a potential hazard should the oxygen levels deplete. The main senor unit is wall mounted and repeater units can be sited at other entrances. The system has a long life O2 sensor, allowing simple calibration with low maintenance.
Andris Grunskis, pictured here, embryologist at AVA, is also the Quality Systems Manager who specified the O2NE. He graduated from the University of Latvia with a Master’s degree in Biology and regularly participates in international conferences and hands-on workshops and is a member of European Society of Human Reproduction and Embryology and the Nordic Fertility Society. " ... your engineers were great!" he commented after installation.
The 8th Congress of the Asia Pacific Initiative on Reproduction (ASPIRE 2018) will be held at Taipei International Convention Center, Taipei, Taiwan from 12 - 15 April 2018. Over the three days, the conference will consider the latest developments and their impact on the future of fertility preservation, embryology, andrology, clinical trials, PCOS, PGS, IVM, and related fields.
Planer will be exhibiting at Aspire 2018. If you are planning to visit the conference, don’t forget to come and see us on stand C53. We will be displaying the full range of Planer IVF products, including the CT37stax™ multi chamber incubator, the DATAssure™ wireless alarm and monitoring system as well as Shipslog3™ and PetriSense®.
The Upper Egypt Assisted Reproduction Summit (UEARS) held in Cairo last February (21-23) saw the participation of 40 international speakers and 90 national ones for three days of scientific discussion with close to 1600 attendees from Egypt and overseas. With seven pre-congress workshops and a Consensus meeting, we looked into the micro environments, that can support the embryo in vivo and in vitro starting from how the human life began, navigating through the current and future techniques, including gene editing, with the current standards of embryo culture in between. New papers from 14 researches were presented for the first time during UEARS from young researchers.
Discussed in some detail was how the current standards of embryo culture have failed yet to make paradigm-shifting changes during the last 20 years, although some improvement has occurred. One of the elegant topics presented in the conference was from Catherine Racowsky of Harvard University who discussed the advantages of blastocyst transfer concluding that there remains a need for a robust evidence to support the superiority of blastocyst transfer compared with the cleavage-stage transfer. The new culture platforms that can improve the IVF outcomes were also discussed in a complete session.
In collaboration with The Egyptian Knowledge Bank, two research methodology workshops were conducted, navigating how reliable research should be robustly conducted and how a well conducted meta-analysis has to be done. These two research methodology workshops were arranged by Professor Ali Mahran from Asyut University and Dr Ola Laurence from the Egyptian Knowledge Bank, and delivered by Dr Léa Gagnon, PhD of Nature Research.
The conference had three parallel arms of infertility running throughout the three days. The gynaecology program arm, which was built by Drs Hazem Abdelghafar, Mohamed Sabry, Mohamed Yahya of Sohag University and Ahmed Nabil of Asyut University. The andrology program arm that was developed by Dr Ali Mahran. The embryology and genetics program arm, which was built by Mohamed Fawzy of Ibnsina and Banon IVF Centres, Egypt. The embryology arm had two parallel halls of lectures during the three days with a pre-congress four workshops. In the main program, Mohamed Fawzy delivered a talk navigating through his latest three publications discussing humidity, oxygen tension and temperature roles in human embryo culture. For the first time in RCT design, it has been shown that omitting humidity from some incubators was done with no robust evidence but with possible harm to the developing embryos and the downstream events.
The UEARS 2018 lectures were generally considered to be of a very high scientific calibre. All UEARS Activities were well organized by the professional company Pure Spot Events Management; the closing remarks were focused on the concept of "no harm must be the goal in all IVF procedures and interventions". There is an open invitation to join UEARS in 2019 (Feb 20-22) which it is hoped will be of even higher quality and design.
Pictured here: Mohamed Fawzy, Scientific organiser of the embryology arm of UEARS, IVF Lab Director of Ibnsina and Banon IVF Centers Egypt
For further information on UEARS, please visit their website:
Over the last year or so there have been reports in the press on problems with samples stored in liquid nitrogen. Cryo storage is such a reliable method that it is often taken for granted - equipment is generally very robust and supply of liquid nitrogen, LN2, is typically very reliable. Patients as well as new entrants to the field are often surprised to learn that both the freezing of a sample, and its long-term storage, can be more complicated than they seem.
Many people, even those working in ART laboratories, remain unaware that specimens in cryogenic storage will suffer damage due to ice recrystallization when their temperature rises above -132°C (the ice transition point of water). And, the smaller the specimen the greater risk it is at, because its low thermal mass will allow its temperature to rise more quickly, and hence follow more closely, the rising external temperature, that of the storage environment. So the risk of ice recrystallization damage increases when comparing cryotubes to straws to vitrified embryo carrier devices.
Quality and Risk Management in the IVF Laboratory
In a definitive textbook on the subject on quality and risk management in the IVF lab, Drs Sharon and David Mortimer (pictured here) provide extensive background on the whole topic including explaining general principles applicable to managing risk in an Assisted Reproduction lab’s cryobank.
In Chapter 8 “Managing Risk” there is a section on “Monitoring storage cryotanks” where they recount a personal experience of dealing with a degrading dewar and, based on that experience, provide a series of recommendations for routine good practice. And as an extension to the book, Dr David Mortimer has provided the following practical advice.
When using liquid storage in small cryotanks (e.g. up to 50 litres):
When using liquid storage in large cryotanks of say 150 litres and more:
When using vapour storage in smaller dewars manual monitoring of the LN2 level is impractical, and a combination of LN2 level and temperature sensors should be used:
Locate the temperature sensor a good distance above the uppermost specimen in storage and have it alarm when the measured temperature falls below -140°C (which is considered to be the limit for safe vapour storage).
At Planer we are keen to promote safe cryo storage. As well as controlled rate freezers we sell vessels, alarm systems, monitoring systems and cryo transport loggers through our distributors available all over the world.
For further information
For Planer Monitoring: https://planer.com/products/alarms-a-monitoring/datassure-laboratory-wireless-alarm-monitoring-system.html
For ‘Quality and Risk Management in the IVF Laboratory’: http://www.cambridge.org/