News 2016

Best practices for the preservation of cells used therapeutically

Prof Allison Hubel and her team at The Biopreservation Core Resource at the University of Minnesota are giving a course on the "Preservation of Cellular Therapies" in September which may be particularly useful for laboratory and manufacturing directors and practitioners. 

The course aims to teach best practices for preservation of cells used therapeutically and is appropriate for managers of cell therapy laboratories, technicians who preserve cells as a part of their daily routine, scientists involved in the development of cell therapies, companies that produce products for the cell therapy space and technicians in repositories that store cells to be used therapeutically; it will be held on Sept 27-28, 2016 in Minneapolis, USA.

The course topics will cover the Fundamentals of cryopreservation, the Development of Protocol, Regulatory issues in cryopreservation and go on to the Design of a storage facility, which containers, reagents and equipment to use. Also covered will be the Emerging issues in preservation of cell therapies, Clinical cell cryopreservation and related Quality control. The Lecturers are David McKenna, MD, Medical Director, Molecular and Cellular Therapeutics Facility, University of Minnesota, Diane Kadidlo, B.S., Director of the Molecular and Cellular Therapeutics Facility, Fran Rabe, MS, ASQ, Director of Quality Assurance, Molecular and Cellular Therapeutics Facility, University of Minnesota, Ian Pope, PhD, MBA, General Director Cryosolutions Group, Brooks Life Sciences and Allison Hubel, PhD, Director, Biopreservation Core Resource, University of Minnesota.

There is a further course on the Preservation of Molecular, Cellular and Tissue Biospecimens on Sept 28-29, 2016.

For further information:
Schedule of lectures, Course 1
Schedule of lectures, Course 2

 

 

Planer controlled freezer installed at the Clinicas Hospital in Paraguay

We recently installed a Kryo 560-16 controlled rate freezer at the Clinicas Hospital in Paraguay, South America. The Oncohematology Pediatric department, led by Sonia Gini, will be using our unit to freeze peripheral blood. The blood cells are separated into various components such as: white blood cells, platelets and plasma using an apheresis machine.

The team from Chaco international, our new distributor in Paraguay, was trained on site whilst the controlled rate freezer was being installed. Their plan is to use the Planer Kryo 560 in the Clinicas Hospital as a ‘Showroom’ for other clinics and hospitals.

To find our more:
Kryo 560-16 Controlled Rate Freezers
Chaco International, Planer Distributor in Paraguay

 

 

Murine Embryology

In vitro fertilisation is used widely in animal husbandry, human IVF and transgenics and stem cell research. A recent paper by Karen Hazzard of the Embryonic Stem Cell and Transgenic Mouse Core at the National Human Genome Research Institute, Bethesda, Maryland describes experiments evaluating different fertilisation rates in two IVF protocols.

Laboratories use IVF as a valuable tool for efficient assisted reproduction in genetically engineered mice and for breeding mice from cryopreserved sperm, which enables them to archive their valuable genetically engineered strains. Over the last few years, several modification protocols for sperm cryopreservation and IVF have been published in an effort to improve the fertilisation rate. Incidental in this study, a BT37 incubator was cited in the publication and used to provide precise incubation conditions for IVF.

Users tend to choose the BT37 for precision but also for convenience – it provides ease of use and a consistent environment.  As a benchtop machine the BT37 can make working with thawed frozen sperm easier. Some recent murine protocols stress that it is important to move the sperm preincubation-drop as little as possible (when adding sperm to the fertilisation-drop) and it is easier to achieve this with a benchtop incubator such as the  BT37 because it can be located right next to the microscope.

More
J Am Assoc Lab Anim Sci. 2014 Nov; 53(6): 641?646
http://planer.com/products/incubators/bt-37.html

 

 

 

 

First British woman gives birth after an ovarian tissue transplant

The photo shows the controlled rate freezer type that was used to pre-freeze a section of ovarian tissue from the first British woman to give birth after an ovarian tissue transplant.

The 33 year old woman had the tissue removed some ten years ago after being diagnosed with a rare type of kidney cancer. The section had to be controlled rate frozen before it could be stored in ultra-cold liquid nitrogen and this was done in a Planer computer controlled freezer.

The woman, then in her 20s, underwent a course of chemotherapy which usually results in premature menopause and infertility. The cancer treatment was successful and nine years later a team from Edinburgh University's MRC Centre for Reproductive Health, led by Prof Richard Anderson, thawed the preserved tissue and re implanted it successfully - so that in mid June 2016 the woman gave birth to a healthy boy after a natural conception.

Over 30 patients around the world have given birth after having ovarian tissue frozen, stored and re-implanted and in most cases the British made Planer controlled rate freezer was used before the tissue was stored in liquid nitrogen.

In this case the doctors removed a section of tissue from one of the woman's two ovaries ten years ago, which contained healthy but immature eggs. This specimen was treated and then frozen down in the Planer controlled rate freezer in a series of cooling steps so that the tissue was in optimum state before being transferred to a liquid nitrogen tank for storage at a temperature around -190 ℃. The cancer treatment was successful and the team carefully thawed the tissue and the pieces, a few millimetres in size, were transplanted on to the existing ovary.

The team at the University of Edinburgh have been researching the technique for more than twenty years. The Planer company, based near London’s Heathrow, has pioneered controlled temperature equipments for cells, embryos and tissue for forty years - installing some ten thousand machines in eighty countries around the world.

Geoffrey Planer commented “This offers hope to thousands of women who need to undergo treatment for cancer - hats off to Professor Anderson and the team in Edinburgh”.

For further information, please contact:-
Adrian Fuller, Sales Director at This email address is being protected from spambots. You need JavaScript enabled to view it.

Jen Middleton, Press Officer, University of Edinburgh at This email address is being protected from spambots. You need JavaScript enabled to view it.

More details about Professor Richard Anderson
http://www.crh.ed.ac.uk/professor-richard-a-anderson/

CO2 Monitoring Sensor launched by Planer at ESHRE

Planer plc today announces the availability of the new PetriSense monitoring system. It provides reliable, continuous monitoring of the critical parameter of carbon dioxide inside benchtop and big box incubators, providing clinicians with an early indication of potential changes in pH. 

Media pH is a critical parameter that affects the development of the embryos. Careful monitoring of pH levels during the culturing process is crucial as a change of as little as 0.1 is sufficient to impair human embryo growth.

The carbon dioxide, CO2, used within incubators is key to pH control. The surrounding growth media used in culturing, buffers pH and means changes in pH are seen later than changes in CO2. So CO2 monitoring can provide early warning of impending changes in pH so that corrective action can be taken earlier. This is what the new Petri Dish sensor does.

Traditional pH meters taking absolute measurements of pH in media can present users with issues concerning sterility and are subject to daily calibration. More recent sensors can give a direct pH reading continuously via identification of fluorescence changes of substrates. While overcoming sterility issues, these can be subject to drift — and they can be expensive and are not portable.

The Planer PetriSense sensor takes advantage of the relationship between CO2 and pH to provide a reliable continuous method for monitoring and early warning of both CO2 changes and hence related pH — overcoming the issues of portability, sterility, expense and drift.

The PetriSense product is launched by Planer at the European Society of Human Reproduction and Embryology (ESHRE) Annual Meeting being held in Helsinki from 3th to 6th July 2016 on stand number C165.

More about the PetriSense CO2 monitor for Incubators

PetriSense options include the Portable Sense version for monitoring CO2, and hence pH, for short periods of time and a Fixed Sense version that can be added into most types of benchtop incubator. This can also be used as an independent alarm system or connected to a laboratory's own comprehensive monitoring and alarm facility to give 24 hour alerts.

Embryologists and researchers have strived to monitor CO2 or pH effectively and consistently within incubators for a number of years, but now the two PetriSense products provide the opportunity to monitor directly the CO2 and infer the pH continuously via a user-friendly, non-invasive system with its own easy to use logging software. 

Geoffrey Planer comments "Effective continuous CO2 monitoring is an essential next step in ensuring consistent IVF success rates, and we’re delighted to be launching this sensor as the first in our PetriSense range".

To find out more about the PetriSense Sensor, please contact us:-
Email: This email address is being protected from spambots. You need JavaScript enabled to view it.
Telephone: +44 1932 755000

Come and see Planer at ESHRE 2016

The European Society of Human Reproduction and Embryology’s (ESHRE) Annual Meeting will be held this year in Helsinki from 3rd to 6th July. The Society now has over 6,000 members from over 110 countries drawn from the medical and scientific community.

A broad range of subjects will be covered during the Meeting’s three days. Session 62, focusing on oocyte and embryo cryopreservation, looks to be of particular interest. Presentations in this session will include:-

  • Oxidative markers in cryopreservation medium of frozen- thawed embryos in In Vitro Fertilization: a possible tool for improved embryo selection?
    Winer Zofnat, Israel

  • Different thawed cleaved embryo transfer strategy for cryopreserved embryos according to mode of cryopreservation? : a prospective randomized study comparing 2 culture medium duration
    Achraf Benammar, France

  • Higher cumulative ongoing pregnancy rate after IVF cycles including a fresh embryo transfer than after freeze-all cycles
    Virginie Barraud-Lange, France

  • Multi-center study demonstrates freeze-all IVF protocols are correlated with higher ongoing pregnancy rates in women of advanced maternal age
    Karen Hunter Cohn, U.S.A.

If you are planning to attend ESHRE, do come and see the Planer team on Stand number C165. We will be showcasing our range of products, including cryogenic freezers, sample tracking software and our laboratory alarm & monitoring systems.

For further information about ESHRE 2016, please visit
https://www.eshre2016.eu

ISSCR 2016

Planer will be exhibiting at the International Society for Stem Cell Research (ISSCR) conference, one of the largest stem cell and regenerative medicine communities in the world, which is being held in San Francisco, USA from 22nd to 25th June 2016.

The Stem Cell market is a rapidly growing area with lots of investment in new research and techniques especially as more and more therapies are now being offered to patients. Planer controlled rate freezers, sample tracking software, cryo storage vessels and alarm/monitoring systems are in regular use in many stem cell facilities around the world and are at the forefront of the latest research. Many of the latest breakthroughs in the stem cell market have used Planer equipment during their development.

If you are attending the show, come and see the Planer team on booth number 1311. Planer will be showcasing a broad range of products, including cryogenic freezers, sample tracking software and our laboratory alarm & monitoring systems.

For further information on ISSCR please us the link below:
http://www.isscr.org/home/annual-meeting/san-francisco-2016

The Euro Tissue Bank and skin freezing

The Euro Tissue bank based at Beverwijk just north of Amsterdam in Holland is the result of an amalgamation of three existing banks: the skin bank founded for the treatment of burns in 1976, the cornea bank and the heart valve bank - both started some ten years later.

The three organisations merged this January and our Dutch distributor has been chosen as their partner of choice for cryogenic needs. Cryo Solutions recently supplied and commissioned a complete new cryogenic installation consisting of an outside bulk tank, vacuum insulated pipe connecting it to two 400L mini tanks which are connected in turn to bio storage vessels. A liquid nitrogen pressure tank feeds the Planer 560 controlled rate freezers which are used mainly for cryo preserving skin.

The whole suite is managed by a panel which allows central management and can be accessed and controlled through a touchscreen.

For more see
http://www.eurotissuebank.nl/euro-tissue-bank/
http://planer.com/products/cryo-freezers/medium-crf/kryo-560.html
http://www.cryosolutions.nl/

 

 

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