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Come and see us at CRYO2018

Cryo2018

Planer will be exhibiting at the 55th Annual Meeting of the Society of Cryobiology (CRYO2018), which will be held from 10-13 July in Madrid, at the Spanish National Research Council, one of the largest research institutes in Europe. The theme of the meeting is ‘Scientific Challenges of Cryobiology’.

Held over four days, the meeting will feature five plenary speakers and more than 25 invited speakers from around the world. There will also be a full programme of submitted abstract oral and poster presentations covering all aspects of cryobiology – from human and animal cells, to plant preservation, to cryosurgery, to food technology, and much more.

If you attending CRYO2018, please do come and see us on our stand, which you can find in the cloisters area of the CSIC campus.

For more information
Cryo2018 website

 

Biobanking in the 21st Century

Biobanking in the 21st CenturyWith Europe Biobank Week 2018 in Antwerp, Belgium coming up (September 4-7th) people will be reviewing the available information on the subject. A definitive book is published by Springer - and of the fourteen chapters, chapter five, "The Future of Cell Preservation Strategies" by John M. Baust et al will probably interest our customers.

Dr Baust introduces this subject: " ... cryopreservation is often viewed as an “old school” discipline yet modern cryopreservation is undergoing another scientific and technology development growth phase. In this regard, today’s cryopreservation processes and cryopreserved products are found at the forefront of research in the areas of discovery science, stem cell research, diagnostic development and personalised medicine. As the utilisation of cryopreserved cells continues to increase, the demands placed on the biobanking industry are increasing and evolving at an accelerated rate. No longer are samples providing for high immediate post-thaw viability adequate. Researchers are now requiring samples where not only is there high cell recovery but that the product recovered is physiologically and biochemically identical to its pre-freeze state at the genominic, proteomic, structural, functional and reproductive levels".

He continues "It is often unappreciated that in order for a cell to be successfully cryopreserved the cell itself must avoid freezing, therefore remaining in a state of deepening hypothermia until glass transition temperature is reached. In essence, for a cell to be successfully cryopreserved, it must remain in a ultra-cold liquidous state until transitioning to a glassy state. Generally speaking, if ice forms within a cell during any part of the process, survival will be compromised. Cryoprotective agents (CPAs) function, in part, to lower the probability of intracellular ice formation.... today's CPAs include a variety of penetrating (membrane permeable) and non-penetrating compounds contained in an appropriate cell culture media with or without serum."

The science of Biobanking, which initially involved simply storing blood or tissue samples in a freezer, is now a highly sophisticated field of research, and expected to grow exponentially over the next decade or two. The book aims to enrich the available literature by offering a useful collection of ideas for the future. It outlines the experiences of developing modern Biobanking repositories in different countries, whilst covering specific topics regarding its many aspects.

For further information
Biobanking in the 21st Century, Springer

Increased follicle survival in frozen–thawed human ovarian tissue


Transplantation with adipose tissue derived stem cells increased follicle survival in frozen–thawed human ovarian tissue. 

A recent paper published in 'Human Reproduction' by Diego Manavella et al looked at a two-step transplantation process using adipose tissue derived stem cells in which they found increased follicle survival by enhancing vascularization in xenografted frozen–thawed human ovarian tissue.

The team from the Gynaecology Research Unit, Institut de Recherche Expérimentale et Clinique, UCL Louvain, who use Planer controlled rate freezers, found that higher rates of oxygenation and vascularization of ovarian tissue, as well as increased follicle survival rates, were detected in the early post-grafting period. The results suggested that the proposed transplantation procedure with ASCs is a promising step towards potentially solving the problem of massive follicle loss after ovarian tissue grafting.

For further information
Article: Human Reproduction, Volume 33, Issue 6, 1 June 2018, Pages 1107–1116,

New International Sales Manager joins Planer

Phil Crow International Sales ManagerWe are delighted to introduce Phil Crow who joined the Planer sales team on 1st June as the International Sales Manager for Asia and the Middle East.

Phil brings to Planer a vast array of experience and knowledge of international distributor management in the healthcare industry, most recently having worked as the National Sales Manager at Apex Medical. He describes himself as a highly motivated and driven sales professional who enjoys the challenge of providing a high level of customer satisfaction.

Phil and his wife Deborah like to spend their down time appreciating good food, live entertainment and the movies, anything from the classics to the latest blockbuster; but most of all, providing he's allowed, Phil enjoys his one escape from the rat race on the golf course. A big fan of fictional crime novels, Phil is currently reading his way through author Simon Kernicks library of fast paced bestsellers.

 

ESHRE 2018 - Come and see us on stand 386


ESHRE 2018 logoPlaner will once again be exhibiting at the European Society of Human Reproduction and Embryology (ESHRE) conference in 2018. This year the conference is being held at the Centre de Convencions Internacional de Barcelona (CCIB) in Barcelona, Spain from the 1st to the 4th July.

We would welcome any of our distributors and their customers to come and see us at this year’s conference where we will have the CT37stax™ multi chamber incubator and DATAssure™alarm and monitoring system on display alongside a range of cryogenic storage vessels suitable for the ART market.

We look forward to seeing many of you at this important event in the ART calendar. Please do let us know if you are planning on attending - we will be on stand 386.

Planer to attend Alpha 2018, Reykjavik, Iceland

This year, the biennial meeting of ALPHA, the international society aimed at advancing the science of clinical embryology around the world, will be taking place in the beautiful city of Reykjavik, Iceland between 17th and 20th May. Planer will be attending the meeting – if you are going, please do come and find out more about our latest range of products.

CT37stax™ multi chamber incubator
Our CT37stax™ incubator complements the existing range of well-established Planer benchtop incubators. It is a high capacity incubator, which has been designed using the best features of the existing technology and incorporates these with a mix of innovative new features to provide a state of art new multi chamber benchtop incubator fit for the demands of the modern laboratory.

We are delighted to announce that the CT37stax™ has just gained medical device accreditation under the Medical Devices Directive 93/42/ECC - which now allows this product to be sold for human applications e.g. ART applications in all countries covered by these regulations.

DATAssure™ wireless monitoring system
The Planer DATAssure™ wireless monitoring system is used across a broad range of market sectors meeting the most stringent standards to assist customers to comply with HACCP, BRC, FDA and MRHA legislative requirements. The system is standalone and connects directly to your business IT network with no need for any dedicated PC, server or specialist installed software. Data from the base station can be viewed direct on the colour touch screen or via a standard web browser.

ShipsLog3™
Our ShipsLog3™ is a temperature data logger that provides an accurate and downloadable temperature history of your vapour shipper throughout its transit. It measures and stores more than 32,000 temperature readings from the thermocouple fitted to the inside of the shipper lid. This will monitor and record the temperature of the shipper at the warmest part ensuring the samples in the core of the shipper are stored at the correct temperature and providing early alerts of potential warming issues.

If you are unable to attend ALPHA, but would like to find out more about our range of products suitable for the clinical embryology field, please do get in touch. We would be delighted to hear from you.

 

 

Freezing Responses in DMSO Based Cryopreservation of Human iPS


A study on these freezing responses, relating to aggregates versus single cells, has just been published in Tissue Engineering (on-line February 2018) by Rui Li, Guanglin Yu (pictured here) and others working with Prof Allison Hubel's team at the Department of Mechanical Engineering University of Minnesota. Human induced pluripotent stem cells (hiPSCs) are multicellular aggregates attracting much interest in tissue engineering, disease modelling and personalised medicine. They can be frozen either as aggregates or single cells depending upon the application. For both clinical and scientific purposes, effective cryopreservation of hiPSCs is required for transportation, storage of frozen hiPSCs and other downstream uses. However, cryopreserved hiPSCs are vulnerable to the loss of viability, function or pluripotency.

It is known that inadequate preservation methods of hiPSCs have impeded efficient re-establishment of cell culture after their freeze-thaw. In the study the roles of cooling rate, seeding temperature and the difference between cell aggregates and single cells in controlled rate freezing were examined using, inter alia, Raman spectroscopy, as a tool for understanding cell responses to the freezing environment. The Raman spectroscopy was used to observe both hiPSC single cells and aggregates frozen at three cooling rates and two seeding temperatures; it suggested higher sensitivity of aggregates to supercooling than previously thought. The work will deepen understanding of behaviours of single cells and aggregates frozen at various conditions and help promote the development of improved cryopreservation protocols for human induced pluripotent stem cells.

For single cells, slow cooling rates allowed significantly better preservation of membrane integrity than higher cooling rate (10˚C/min) regardless of the seeding temperature. For aggregates, however, slow cooling rates (1, 3˚C/min) combined with high seeding temperature (4˚C) had little effect on the membrane integrity but resulted in significantly better cell attachment than higher cooling rate (10˚C/min) or low seeding temperature (8˚C). The authors say there are advantages of using a seeding temperature of 4˚C compared to 8˚C suggesting that the range of seeding temperatures of 7˚C to 12˚C quoted in much literature may be sub-optimal, and that seeding temperature should be considered as a critical parameter when designing cryopreservation protocol for hiPSCs. Guanglin Yu, see photo above, carried out a lot of the experimentation. He said "...we used manual seeding for nucleation of the sample. We sprayed the sample with a narrow stream of liquid nitrogen. We saw different cell responses of HiPSC with seeding temperature of -4˚C and -8˚C using Raman microscopy. This made it clear that we wanted to control the temperature at which ice formed in the extracellular space for controlled rate freezing experiments."

The paper indicates that hiPSCs respond to freezing in very complex fashion, and successful establishment of post thaw culture depends on various critical factors. Further studies will need to not only continue exploring additional factors to optimise the freezing protocol for hiPSCs but investigate the biological pathways connecting the factors and the observed cryopreservation outcomes to provide targets for future development of cryoprotectants.

For further information
Contact Prof Hubel's Lab: This email address is being protected from spambots. You need JavaScript enabled to view it.
Tissue Engineering: https://www.liebertpub.com/doi/abs/10.1089/ten.TEC.2017.0531
Planer Controlled Rate Freezer: https://planer.com/products/cryo-freezers/medium-crf.html

The first frozen embryo baby

On the 34th anniversary of the birth of the first baby from a frozen embryo, the BBC World Service broadcast an interview with Alan Trounson the pioneer doctor who undertook the procedure.

Zoe Leyland was born in Melbourne, Australia on 28th March 1984, helped on her way by Drs Trounson and Wood who made medical history. The decision to try 'test tube' fertilisation and embryo freezing was taken by Zoe's parents – her mother a 33 year old New Zealander and father a 38 year old British born Australian resident. Her mother had hormonal stimulation and produced eleven eggs which were frozen using a then new type of controlled rate freezer made by Planer plc. One of those frozen embryos became Zoe - who weighed in at about 5 lbs or 2.5 kilos.

The world's first 'fresh’ test tube baby was Louise Brown born in England in 1978, but Zoe came from an embryo frozen for a time before being thawed and implanted. To allow cells to survive liquid nitrogen temperatures (-196°C) the embryos had to be treated with cryo-protectant, then slowly frozen down in the Planer freezer with extreme precision using different temperature ramps, before being stored in liquid nitrogen. This controlled rate freezing procedure is now used in cell laboratories worldwide. The controlled rate freezing technique, originally suggested some fifty years ago by British Scientist Professor David Pegg, enabled Planer plc to pioneer the equipment and today many thousands of units are in constant use all over the world in stem cell labs, IVF, hospitals and research institutions. This controlled rate freezing is often needed before storing certain types of cells in liquid nitrogen – in areas such as cord blood banking, bone marrow transplants, botanical matter, semen, oocytes, botanical seeds, skin, ovarian tissue, heart valves and blood vessels.

For further information:
BBC World Service: The first frozen embryo baby
Forty years on ...Planer freezers


News Stories - 2018

News Stories - 2017